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KMID : 1140120120170020128
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Cancer Prevention Research
2012 Volume.17 No. 2 p.128 ~ p.140
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Antioxidative and Antigenotoxic Effect of Schizandra chinensis B. Extracted with Water at Various Temperatures
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Kim Min-Jung
Park Eun-Ju
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Abstract
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Schizandra Chinensis B. (SC) was used as traditional edible and medicinal materials in East Asia. In this study, the antioxidant effect of SC extracted with water at various temperatures (25oC; SC25, 50oC; SC50, and 90oC; SC90) were investigated by measuring DPPH radical-scavenging activity (RSA), superoxide dismutase (SOD)-like activity, oxygen radical absorbance capacity (ORAC), and cellular antioxidant capacity (CAC) assay. The effect of SC extract on DNA damage induced by H2O2 in human leukocytes was evaluated by comet assay. The total phenolic content (TPC) of SC90, SC50 and SC25 were 547.2, 553.7 and 479.3 mg/100 g gallic acid equivalents, respectively. The IC50 for DPPH RSA was in order of SC90 (1672.9 μg/ml)=SC50 (1674.6 μg/ml)＞SC25 (1860.6 μg/ml). Water extract of SC showed the IC50 for SOD-like activities 886.1, 980.2 and 1003.9 μg/ml in SC90, SC50 and SC25, respectively. The ORACROOㆍ value of SC extract (at 50 μg/ml) is 5.0 μM of TE when extracted at 50oC. The CAC against AAPH－ induced oxidative stress in HepG2 cells was effectively protected by all of the SC extracts. The IC50 value of SC90 has shown the highest inhibition (31.4 μg/ml) on H2O2 induced DNA damage, followed by SC50 (35.8 μg/ml) and SC25 (48.1 μg/ml). These results suggest that water extract of SC possesses a significant antioxidant activity and protective effect against oxidative DNA damage and these properties were more effective when it is extracted at the temperature of 90oC.
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KEYWORD
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Schizandra Chinensis B. water extract, Antioxidant activity, ORAC, Antigenotoxic effect
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